DILUTION OF DIBROM® FOR THE BOTTLE BIOASSAY

DRAFT DOCUMENT FOR REVIEW

PHEREC Technical Memorandum # 4.

The active ingredient in Dibrom® is Naled.

1.  Start with the label.
2.  1 gallon of DIBROM® contains 14.1 pounds of Naled.
3.  Convert pounds per gallon to grams per milliliter.
4. 14.1 lbs/ 1 gallon  X  453.6 grams/ 1 lb X 1 gallon/3785 ml  = 1.7 grams of Naled / ml of DIBROM®.

5. Take 1 ml of DIBROM® and put it in a 100 ml volumetric flask.
6. Fill to 100 ml with A.C.S. acetone; swirl to mix.
7. This is the STOCK SOLUTION.

8. Prepare the TEST SOLUTION as follows:
9. Take 0.147 ml of the STOCK SOLUTION and put it in a 100 ml volumetric flask.
10. Fill to 100 ml with A.C.S. acetone; swirl to mix.
11. This is the TEST SOLUTION.

Measure 1 ml of the TEST SOLUTION into each 250 ml Wheaton bottle for the bioassay.

This procedure will yield 25 µg of Naled in each Wheaton bottle for the bioassay.